With the development of nanotechnology, functionalized gold nanoparticles have been widely used in bioanalysis and medical detection due to their unique photoelectric properties. However, the gold nanoparticles prepared by traditional chemical reduction methods have the problems of poor stability and high toxicity. Using microbial assisted biosynthesis of gold nanoparticles, green products can be obtained. On the other hand, the surface of the gold nanoparticles needs to be modified to achieve specific recognition. It is difficult to attach antibodies or DNA probes directly on the surface of gold nanoparticles. If the gold nanoparticles with uniform mass and negative surface charge can be obtained, it will be beneficial to the subsequent biological probe connection.
Therefore, by modifying the surface of commercial yeast to prepare high-quality gold nanoparticles, and studying their application in biological detection, this study can enable students to have a deeper understanding of functional nanomaterials, exercise experimental skills, and also have certain scientific research and innovation significance.
Project Name:
A novel strategy for targeted therapy of tumor stem cells based on CRISPR/Cas9 technology
Project background:
Tumor stem cells are important factors leading to tumor occurrence, recurrence and metastasis. The targeted therapy of tumor stem cells is a hot and difficult topic in current tumor therapy research.
Brief introduction of the project:
CRISPR/Cas9 technology was used for gene editing of tumor stem cell specific markers to study their mechanism of action in tumor development and develop new strategies for tumor stem cell targeted therapy.
Identify stem cell markers for different tumor types; Use CRISPR/Cas9 technology to knock out or activate these marker genes; To observe the effects of gene editing on proliferation, differentiation, apoptosis, invasion and metastasis of tumor stem cells. The specific markers of tumor stem cells were used to design targeted vectors and develop targeted therapeutic strategies for tumor stem cells.
Project content:
● Literature research collected tumor stem cell markers;
● Designed sgRNA and constructed CRISPR/Cas9 editing plasmid;
● Cell culture, transfection and identification;
● Establish tumor model and evaluate drug efficacy.
Topic innovation:
● Use CRISPR/Cas9 technology to study the function of tumor stem cell markers;
● Develop targeted therapeutic strategies based on tumor stem cell markers.
Research objectives:
CRISPR/Cas9 technology is used to elucidate the function of tumor stem cell markers and provide theoretical basis and new strategies for tumor targeted therapy.
Research results Value:
Increase your influence and recognition, improve your own series of soft strengths in addition to standardized test scores, fully demonstrate your academic ability to help study abroad applications, improve the competitiveness of comprehensive evaluation and strong base program, so that you can stand out from the fierce competition and directly recommend to elite schools, and get the favor of these top schools! Suitable for people: comprehensive evaluation, strong foundation plan, talent plan, overseas study, early establishment of a high-value, credible, persuasive comprehensive quality evaluation file for students, which will be of great benefit to students' future entrance to the ideal university and future growth path.